- The major research interests of the team LOMONTE are:
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- The study of the pathological consequences of the infection of neurons from the peripheral and central nervous systems by the neurotropic virus herpes simplex virus 1 (HSV-1). We essentially focus our research on the establishment of latency and reactivation processes.
- The study of the implication of nuclear architecture, especially promyelocytic leukemia nuclear bodies (PML NBs), in the regulation of latent HSV-1 chromatin features and in chromatin dynamics during the process of senescence.
- The study of the chromatin alterations associated to damage or to genetic pathologies, and potentially linked to genetic instabilities, or motoneurons decay.
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TEAM
- Patrick LOMONTE
SENIOR RESEARCHER CNRS - Tristan BARGAIN
PHD STUDENT - Faouzi BAKLOUTI
SENIOR RESEARCHER INSERM - Lucas BONNEFOY
Student - Florent BRESSAC
POST-DOC - Armelle CORPET
ASSOCIATE PROFESSOR UCBL - Tristan ESCURE
PHD STUDENT - Clémence GUERRIAU
RESEARCH ASSISTANT - Franceline JUILLARD
ASSISTANT PROFESSOR at SupBiotech - Rémi LEBON
Master 2 student - Chloé MADRAS
RESEARCH ASSISTANT - Karine MONIER
RESEARCH ENGINEER CNRS - Rémi NEPLAZ
PhD student - Delphine PONCET
PROFESSOR UCBL
ALUMNI
- Wilhelm BOUCHEREAU
POST-DOC - Lucien LEROND
Master 2 student - Mathilde GONIN
RESEARCH ASSISTANT - Coline SEURRE
RESEARCH ASSISTANT - Axel FONTANIER
UNDERGRADUATE STUDENT - Corentin ROUSSET
RESEARCH ASSISTANT CNRS (CDD) - Hana TRIKI
POST-DOC - Simon ROUBILLE
PHD STUDENT - Pascale TEXIER
RESEARCH ASSISTANT CNRS - Olivier BINDA
POST-DOC - Clément DROILLARD
POST-DOC - Constance KLEIJWEGT
PHD STUDENT - Emeline TURQUER
Master 2 STUDENT - Aleth CALLE
Research Assistant - Camille COHEN
PhD student - Indri ERLIANDRI
Post-Doc - Karine JACQUET
Post-Doc - Aimé-Boris KIMENYI-ISHIMWE
Master 2 Student - Mohamed-Ali MAROUI
Post-doc
PROJECTS
Beside the study of the biology of HSV-1, one of the main focus of the team is to understand, at the molecular level and using HSV-1 as a model, some basic aspects of nuclear and chromatin dynamics related to the maintenance of genome integrity and to the regulation of gene expression. (See web site for more details)
SELECTED PUBLICATIONS
- Lahaye X, Tran Van P, Chakraborty C, Shmakova A, Cao NTB, Ferran H, Ait-Mohamed O, Maurin M, Waterfall JJ, Kaufer BB, Fischer P, Hennig T, Dölken L, Lomonte P, Fachinetti D, Manel N. 2025. Centromeric DNA amplification triggered by viral proteins activates nuclear cGAS. Cell 2025 May 29:S0092-8674(25)00556-2
- Rousset C, Neplaz R, Catal P, Chatre E, Place C, Lomonte P, Juillard F, Favier A et Monier K. 2025. Correlation-based nanometric localization using lattice SIM2 and dSTORM in PML nuclear bodies validated by calibration spheres. Biochemical and Biophysical Research Communications Volume 752, 8 March 2025, 151450
- Roubille S, Escure T, Juillard F, Corpet A, Néplaz R, Binda O, Seurre C, Gonin M, Bloor S, Cohen C, Texier P, Haigh O, Pascual O, Ganor Y, Magdinier F, Labetoulle M, Lehner PJ, Lomonte P. 2024. The HUSH epigenetic repressor complex silences PML nuclear body-associated HSV-1 quiescent genomes Proc Natl Acad Sci USA 121:e2412258121
- Berthenet K., Aïmontché E., El Mrini S., Brière J., Pion N., Iacono I., Brejon S., Karine Monier K., Frédéric Catez F., Ichim G., Combaret V., Hichem C. Mertani H.C., Diaz J.J. and Albaret M.A. 2024. Spatial sequestration of activated-caspase 3 in aggresomes mediates resistance of neuroblastoma cell to bortezomib treatment, Sci Rep 14, 3768
- Kleijwegt C, Bressac F, Seurre C, Bouchereau W, Cohen C, Texier P, Simonet T, Schaeffer L, Lomonte P, Corpet A. 2023. Interplay between PML NBs and HIRA for H3.3 dynamics following type I interferon stimulus. eLife 12:e80156. doi:10.7554/eLife.80156
- Binda, O., Kimenyi Ishimwe, AB., Galloy, M., Jacquet, K., Corpet, A., Fradet-Trucotte, A., Côté, J., and Lomonte, P. (2023). The TUDOR domain of SMN is an H3K79 me1 histone mark reader Life Sci Alliance 6:e202201752. doi:10.26508/lsa.202201752
- Binda, O., Juillard, F., Ducassou J.N., Kleijwegt, C., Paris G., Didillon A., Baklouti F., Corpet, A., Couté, Y., Côté, J. and Lomonte, P. (2023). SMA-linked SMN mutants prevent phase separation properties and SMN interactions with FMRP family members. Life Sci Alliance 6:e202201429. doi:10.26508/lsa.202201429
- Jacquier A, Roubille S, Lomonte P, Schaeffer L. (2022). Microrchidia CW-Type Zinc Finger 2, a Chromatin Modifier in a Spectrum of Peripheral Neuropathies. Front Cell Neurosci. Jun 3;16:896854. doi: 10.3389/fncel.2022.896854. eCollection 2022.
- Rousseau A, Haigh O, Legrand R, Palgen JL, Lemaitre J, Deback C, Oziol N, Lomonte P, Labetoulle M. (2022). Initial TK-deficient HSV-1 infection in the lip alters contralateral lip challenge immune dynamics. Sci Rep. May 19;12(1):8489. doi: 10.1038/s41598-022-12597-4.
- Billard P, Guerriau C, Carpentier C, Juillard F, Grandin N, Lomonte P, Kantapareddy P, Dufay N, Barritault M, Rimokh R, Verrelle P, Maucort-Boulch D, Figarella-Branger D, Ducray F, Dehais C, Charbonneau M, Meyronet D, Poncet DA; POLA network. (2021). The TeloDIAG: how telomeric parameters can help in glioma rapid diagnosis and liquid biopsy approaches. Ann Oncol. Dec;32(12):1608-1617. doi: 10.1016/j.annonc.2021.09.004.
- Corpet, A., Kleijwegt, C., Roubille, S., Juillard, F., Jacquet, K., Texier, P., and Lomonte, P. (2020). PML nuclear bodies and chromatin dynamics: catch me if you can! Nucleic Acids Research 389, 251–23
- Lomonte, P., Baklouti, F., & Binda, O. (2020). The Biochemistry of Survival Motor Neuron Protein Is Paving the Way to Novel Therapies for Spinal Muscle Atrophy. Biochemistry. Apr14;59(14):1391-1397.
- Marcocci ME, Napoletani G, Protto V, Kolesova O, Piacentini R, Li Puma DD, Lomonte P, Grassi C, Palamara AT, De Chiara G. (2020). Herpes Simplex Virus-1 in the Brain: The Dark Side of a Sneaky Infection. Trends in Microbiology. 1-13.
- Cohen, C., Corpet, A., Maroui, M.A., Juillard, F., and Lomonte, P. (2020). Latent/Quiescent Herpes Simplex Virus 1 Genome Detection by Fluorescence In Situ Hybridization (FISH). Methods Mol. Biol. 2060, 185–197.
- Provost A., Rousset C.,, Bourdon L., Mezhoud S., Reungoat E., Fourneaux C., Bresson T., Pauly M., Béard N., Possi-Tchouanlong L., Grigorov B., Bouvet P., Diaz J.J., Chamot C., Pécheur E.-I., Ladavière C. *, Charreyre M.-T. *, Favier A., Place C.*, Monier K. *. (2019). Innovative particle standards and long-lived imaging for 2D and 3D dSTORM. Sci. Rep. 9(1):17967
- Grandin N, Pereira B, Cohen C, Billard P, Dehais C, Carpentier C, Idbaih A, Bielle F, Ducray F, Figarella-Branger D, Delattre JY, Sanson M, Lomonte P, Poncet D, Verrelle P, Charbonneau M; POLA network. (2019). The level of activity of the alternative lengthening of telomeres correlates with patient age in IDH-mutant ATRX-loss-of-expression anaplastic astrocytomas.Acta Neuropathol Commun. 7, 175.
- Cohen, C., Corpet, A., Roubille, S., Ali Maroui, M.A., Poccardi, N., Rousseau, A., Kleijwegt, C., Binda, O., Texier, P., Sawtell, N., Labetoulle, M., and Lomonte, P. (2018). Promyelocytic Leukemia (PML) Nuclear Bodies (NBs) induce latent/quiescent HSV-1 genomes chromatinization through a PML NB/Histone H3.3/H3.3 Chaperone axis. Plos Pathogens 14(9): e1007313.
FUNDING
- AFM-Téléthon / MyoNeurALP2 (2022-2027)
- ANR Epi-IFN-IM (ANR-21-CE17-0018) (2021-2024)
- CNRS prematuration program: dSTORM imaging in the green channel to reconstruct 3 colors and 3D in super-resolution dSTORM (18 months : 2023-2024)
- ANR CHROMACoV (ANR-20-COV9-0004) (2020)
- ANR EPIPRO (ANR-18-CE15-0014-01) (2018-2021)
- LabEX DEV2CAN (ANR-10-LABX-61)
- LabEX DEVweCAN (ANR-10-LABX-61)
- AFM-Téléthon / MyoNeurALP Alliance (2016-2021)
- France Alzheimer
- Fondation pour la Recherche Médicale (FRM)
- Ligue Contre le Cancer (Comité du Rhône)
- UCBL: CNMD (Ottawa, Canada)-INMG Joint collaborative Research Program
