Phosphatidylinositol 3-kinase inhibition restores Ca2+ release defects and prolongs survival in myotubularin-deficient mice

Candice Kutchukian, Mirella Lo Scrudato, Yves Tourneur, Karine Poulard, Alban Vignaud, Christine Berthier, Bruno Allard, Michael W. Lawlor, Ana Buj-Bello, and Vincent Jacquemond

Myotubular myopathy is a fatal disease resulting from deficiency in the phosphoinositide phosphatase MTM1. Kutchukian et al. (2016) have now identified critical pathophysiological alterations of Ca2+ signaling which are involved in the disease and have revealed the benefit of a pharmacological treatment.

Molecular Cell

The Flexible Ends of CENP-A Nucleosome Are Required for Mitotic Fidelity.

Roulland Y, Ouararhni K, Naidenov M, Ramos L, Shuaib M, Syed SH, Lone IN, Boopathi R, Fontaine E, Papai G, Tachiwana H, Gautier T, Skoufias D, Padmanabhan K, Bednar J, Kurumizaka H, Schultz P, *Angelov D, *Hamiche A, *Dimitrov S.

CENP-A nucleosomal ends are highly flexible in solution, which prevents recruitment of the linker histone H1 thus allowing assembly of active kinetochore complex. Open CENP-A nucleosomal structure is essential for its mitotic function.

IMMUNITY   Communiqué de presse du CNRS   Science & santé

Macrophage PPARgamma, a Lipid Activated Transcription Factor Controls the Growth Factor GDF3 and Skeletal Muscle Regeneration.

Tamas Varga, Rémi Mounier, Andreas Patsalos, Péter Gogolák, Matthew Peloquin, Attila Horvath, Attila Pap, Bence Daniel, Gergely Nagy, Eva Pintye, Szilárd Poliska, Sylvain Cuvellier, Sabrina Ben Larbi, Brian E. Sansbury, Matthew Spite, Chester W. Brown, Bénédicte Chazaud, Laszlo Nagy

PPARγ is a lipid activated transcription factor essential for fat cells and linked to lipid processing in macrophages. Varga et al. (2016) now show that PPARγ is transcriptionally active and regulates the growth factor Gdf3 in repair macrophages in regenerating skeletal muscle, forming a paracrine axis connecting macrophages to muscle progenitor fusion.

Chromatin associated mechanisms in base excision repair – nucleosome remodeling and DNA transcription, two key players.

Menoni H, Di Mascio P, Cadet J, Dimitrov S, Angelov D.

Free Radic Biol Med 2016 Dec 20. pii: S0891-5849(16)31126-1.

Evidence for ARGONAUTE4-DNA interactions in RNA-directed DNA methylation in plants.

Lahmy S, Pontier D, Bies-Etheve N, Laudié M, Feng S, Jobet E, Hale CJ, Cooke R, Hakimi MA, Angelov D, Jacobsen SE, Lagrange T.

Genes Dev 2016 Dec 1;30(23):2565-2570

Extra views on Structure and dynamics of DNA loops on nucleosomes studied with molecular simulations.

Pasi M, Angelov D, Bednar J, Dimitrov S, Lavery R

Nucleus 2016 Nov;7(6):554-559

Real-Time Tracking of Parental Histones Reveals Their Contribution to Chromatin Integrity Following DNA Damage.

Salomé Adam, Juliette Dabin, Odile Chevallier, Olivier Leroy, Céline Baldeyron, Armelle Corpet, Patrick Lomonte, Olivier Renaud, Geneviève Almouzni, and Sophie E. Polo

Molecular Cell 2016 Oct 6; 64(1): 65–78.

Extra views on Structure and dynamics of DNA loops on nucleosomes studied with molecular simulations.

Pasi M, Angelov D, Bednar J, Dimitrov S, Lavery R

Nucleus 2016 Nov 22

Latency Entry of Herpes Simplex Virus 1 Is Determined by the Interaction of Its Genome with the Nuclear Environment.

Mohamed Ali Maroui, Aleth Callé , Camille Cohen, Nathalie Streichenberger, Pascale Texier, Julie Takissian, Antoine Rousseau, Nolwenn Poccardi, Jérémy Welsch, Armelle Corpet, Laurent Schaeffer, Marc Labetoulle, Patrick Lomonte

PLoS Pathogens 2016 Sep 12;12(9):e1005834

mTOR inactivation in myocardium from infant mice rapidly leads to dilated cardiomyopathy due to translation defects and p53/JNK-mediated apoptosis.

Laetitia Mazelin, Baptiste Panthu, Anne-Sophie Nicot, Edwige Belotti, Lionel Tintignac, Geoffrey Teixeira, Qing Zhang, Valérie Risson, Dominique Baas, Emilie Delaune, Geneviève Derumeaux, Daniel Taillandier, Théophile Ohlmann, Michel Ovize, Yann-Gaël Gangloff, Laurent Schaeffer

Journal of Molecular and Cellular Cardiology 2016 Aug;97:213-25.

FACT Assists Base Excision Repair by Boosting the Remodeling Activity of RSC.

Charles Richard JL, Shukla MS, Menoni H, Ouararhni K, Lone IN, Roulland Y, Papin C, Ben Simon E, Kundu T, Hamiche A*, Angelov D*, Dimitrov S*

PLoS Genetics 2016 Jul 28;12(7):e1006221.

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).

Klionsky DJ et al.

Autophagy 2016 12(1):1-222.

Highly Dynamic Transcriptional Signature of Distinct Macrophage Subsets during Sterile Inflammation, Resolution, and Tissue Repair.

Varga T, Mounier R, Horvath A, Cuvellier S, Dumont F, Poliska S, Ardjoune H, Juban G, Nagy L, Chazaud B.

Journal of Immunology 2016 Jun 1;196(11):4771-82.

NFκB is a central regulator of protein quality control in response to protein aggregation stresses via autophagy modulation.

Nivon M, Fort L, Muller P, Richet E, Simon S, Guey B, Fournier M, Arrigo AP, Hetz C, Atkin JD, Kretz-Remy C.

Mol Biol Cell. 2016 Jun 1;27(11):1712-27.

Notch Stimulates Both Self-Renewal and Lineage Plasticity in a Subset of Murine CD9High Committed Megakaryocytic Progenitors.

Weiss-Gayet M, Starck J, Chaabouni A, Chazaud B, Morlé F.

PLoS One 2016 Apr 18;11(4):e0153860.

Inflammation during skeletal muscle regeneration and tissue remodeling: application to exercise-induced muscle damage management.

Chazaud B

Immunol Cell Biol. 2016 Feb;94(2):140-5.

Instructive role of M-CSF on commitment of bipotent myeloid cells involves ERK-dependent positive and negative signaling.

Carras S, Valayer A, Moratal C, Weiss-Gayet M, Pages G, Morlé F, Mouchiroud G, Gobert S.

J Leukoc Biol. 2016 Feb;99(2):311-9.