We are pleased to welcome Paolo Grumati from the Telethon Institute of Genetics and Medicine (TIGEM) on February 12th who is invited by Flavie STRAPPAZZON.
He will give a seminar at 2 pm entitled: “ER-phagy regulates ER dynamics during myogenesis”.
The seminar will be in the Amphi 2, 2nd floor.
Abstract:
Endoplasmic reticulum (ER) plasticity and ER-phagy are intertwined processes essential for maintaining ER dynamics. We investigated the interplay between two isoforms of the ER-phagy receptor FAM134B in regulating ER remodeling in differentiating myoblasts. During myogenesis, the canonical FAM134B1 is degraded, while its isoform FAM134B2 is transcriptionally upregulated. The switch, favoring FAM134B2, is an important regulator of ER morphology during myogenesis. FAM134B2 partial reticulon homology domain, with its rigid conformational characteristics, enables an efficient ER reshaping. FAM134B2 action increases in the active phase of differentiation leading to ER restructuring via ER-phagy, which then reverts to physiological levels when myotubes are mature and the ER is reorganized. Knocking out both FAM134B isoforms in myotubes results in an aberrant proteome landscape and the formation of dilated ER structures, both of which are rescued by FAM134B2 re-expression. Our results underscore how the fine tuning of FAM134B isoforms and ER-phagy orchestrate the ER dynamics during myogenesis providing insights into the molecular mechanisms governing ER homeostasis in muscle cells.
Research Papers & Reviews
Number of publications in peer reviewed journals: 61. H Index: 36 (Scopus)
The full list of publications can be retrieved in the next link: https://pubmed.ncbi.nlm.nih.gov/?term=grumati+p
Selected papers:
| 1. | Two FAM134B isoforms differentially regulate ER dynamics during myogenesis.
Buonomo V, Lohachova K, Reggio A, Cano-Franco S, Cillo M, Santorelli L, Venditti R, Polishchuk E, Peluso I, Brunello L, Cirillo C, Petrosino S, Silva M, De Cegli R, Di Bartolomeo S, Gargioli C, Swuec P, Cortese M, Stolz A, Bhaskara RM, Grumati P. EMBO J. 2025 Jan 6. doi: 10.1038/s44318-024-00356-2. Online ahead of print. PMID: 39762646 |
| 2. | The function of ER-phagy receptors is regulated through phosphorylation-dependent ubiquitination pathways.
Berkane R, Ho-Xuan H, Glogger M, Sanz-Martinez P, Brunello L, Glaesner T, Kuncha SK, Holzhüter K, Cano-Franco S, Buonomo V, Cabrerizo-Poveda P, Balakrishnan A, Tascher G, Husnjak K, Juretschke T, Misra M, González A, Dötsch V, Grumati P, Heilemann M, Stolz A. Nat Commun. 2023 Dec 15;14(1):8364. doi: 10.1038/s41467-023-44101-5. PMID: 38102139 |
| 3. | Cancer cells adapt FAM134B/BiP mediated ER-phagy to survive hypoxic stress.
Chipurupalli S, Ganesan R, Martini G, Mele L, Reggio A, Esposito M, Kannan E, Namasivayam V, Grumati P, Desiderio V, Robinson N. Cell Death Dis. 2022 Apr 18;13(4):357. doi: 10.1038/s41419-022-04813-w. PMID: 35436985 |
| 4. | Role of FAM134 paralogues in endoplasmic reticulum remodeling, ER-phagy, and Collagen quality control.
Reggio A, Buonomo V, Berkane R, Bhaskara RM, Tellechea M, Peluso I, Polishchuk E, Di Lorenzo G, Cirillo C, Esposito M, Hussain A, Huebner AK, Hübner CA, Settembre C, Hummer G, Grumati P, Stolz A. EMBO Rep. 2021 Sep 6;22(9):e52289. doi: 10.15252/embr.202052289. Epub 2021 Aug 2. PMID: 34338405 |
| 5. | Phosphorylation of FAM134C by CK2 controls starvation-induced ER-phagy.
Di Lorenzo G, Iavarone F, Maddaluno M, Plata-Gómez AB, Aureli S, Quezada Meza CP, Cinque L, Palma A, Reggio A, Cirillo C, Sacco F, Stolz A, Napolitano G, Marin O, Pinna LA, Ruzzene M, Limongelli V, Efeyan A, Grumati P, Settembre C. Sci Adv. 2022 Sep 2;8(35):eabo1215. doi: 10.1126/sciadv.abo1215. Epub 2022 Aug 31. PMID: 36044577 |